固相萃取法和QuEChERS法对比检测无核荔枝中19种农药残留,本论文主要论述了固相萃取论文范文相关的参考文献,对您的论文写作有参考作用。
固相萃取论文参考文献：
摘 要 建立了气相色谱-串联质谱（GC-MS/MS）法同时测定无核荔枝中19种农药残留的分析方法.实验考察了固相萃取法和QuEChERS法中影响净化和萃取效果的各个因素,同时对二者的基质效应进行分析.结果表明：19种农药的线性范围在0.01～0.5 μg/mL,相关系数均大于0.99.在0.01、0.1、0.5 mg/kg加标水平下,采用固相萃取净化时,19种农药的平均回收率为67.0%～95.6%,RSD为2.5%～12%.采用QuEChERS净化时,19种农药的平均回收率在68.2%～125%,RSD为2.8%～12.7%.2种方法在灵敏度和精密度方面无显著差异,均符合农药残留分析要求.但固相萃取较QuEChERS基质效应小,净化相对彻底.QuEChERS较固相萃取具有简单、快速、环保等优点.2种方法均能对荔枝样品进行检测,且数据准确可靠.
关键词 气相色谱-串联质谱（GC-MS/MS）；固相萃取（SPE）；QuEChERS；农药；残留；无核荔枝
中图分类号 S667.1 文献标识码 A
Determination of 19 Pesticides Residues in Seedless-Litchi by
Gas Chromatography-Tandem Mass Spectrometry
LI Pingping, ZHANG Yue, WU Xiaofang, YANG Xinfeng*, ZHANG Zhenshan
Laboratory of Quality & Safety Risk Assessment for Tropical Agro-Products, Ministry of Agriculture / Analysis
and Testing Center, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China
Abstract Two methods of sample preparation were developed for simultaneous analysis of 19 pesticides residues in seedless-litchi in the study. Factors affecting purification and extraction efficiency in solid phase extraction（SPE）and QuEChERS methods were investigated and optimized. Matrix effects of two methods were analyzed. The results showed that the correlation coefficients were above 0.99 when the concentration was ranged from 0.01 to 0.5 μg/mL. For SPE cleanup method, when the fortified levels from 0.01 to 0.5 mg/kg, the average recoveries were ranged from 67.0% to 95.6% and the relative standard deviations（RSD）were ranged from 2.5% to 12%. For QuEChERS cleanup method, the fortified recoveries was at 0.01, 0.1 and 0.5 mg/kg were in the range of 68.2%-125%, with relative standard deviations（RSD）not more than 12.7%. All the above observations indicated that the two methods were no significant difference in sensitivity and accuracy and suitable for the determination of 19 pesticides in seedless-litchi. Besides, SPE method has the characteristics of relatively thorough purification and week matrix effect. QuEChERS cleanup is more simple, quick and green than SPE. The two methods were accurate and reliable for simultaneous determination of multiple pesticide residues in seedless-litchi.
Key words Gas chromatography-tandem mass spectrometry（GC-MS/MS）； solid phase extraction（SPE）； QuEChERS； pesticides； residue； seedless-litchi
doi 10.3969/j.issn.1000-2561.2017.08.030
荔枝（Litchi chinensis）,屬于无患子科植物,是热带、南亚热带气候区的特种水果,被誉为“果中之王”.中国是世界上最大的荔枝生产国,栽培历史悠久[1-3].通过实生选种和芽变选种已选育出了没有果核、个大汁多味甜的无核荔枝,受到消费者的追捧[4].目前农药被广泛应用于杀灭作物病虫害,但农药大量使用甚至滥用也会导致荔枝果品严重污染,农药残留超标.为控制农药使用量,世界各国和国际组织均制定了农药中的最大残留限量值（MRLs）[5].如CAC规定了200多种农药的MRLs；中国GB 2763-2016《食品中农药最大残留限量》也规定了417种农药的MRLs.因此开发灵敏度高、重现性好、可用于荔枝中多种农药残留的检测方法,不仅能够应对日益增多的农药检测项目,提高工作效率,更重要的是加强荔枝中农药污染的监测和监管,对保障农产品质量安全具有十分重要的意义.
结论：关于本文可作为相关专业固相萃取论文写作研究的大学硕士与本科毕业论文固相萃取小柱论文开题报告范文和职称论文参考文献资料。
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