不同种植方式下烤烟根系差异表达蛋白质分析,关于免费烤烟论文范文在这里免费下载与阅读,为您的烤烟相关论文写作提供资料。
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摘 要：通过根系差异蛋白质组学探讨了种植方式（复种连作、复种轮作）对烤烟生长的影响.结果表明,共有15个蛋白质表达丰度发生变化,用LC-MS/MS鉴定了14个差异蛋白质点,经生物信息学分析,11个蛋白质的功能有注释,其中3个属于和香气形成有关的蛋白质,包括转酮醇酶、单脱氢抗坏血还原酶和O- 转移酶,它们在复种轮作条件下均上调表达,此可能有助于烟叶品质提高,而复种连作处理下调表达,可能不利于品质提高.还鉴定到涉及能量、抗性、物质运输、核酸等功能的蛋白：磷酸甘油酸酯激酶、线粒体内膜移位酶亚基Tim13、NBS-LRR、DnaJ、细胞内囊泡运输蛋白Sly1、核糖体蛋白质,在复种轮作下也上调表达,此可能促进烤烟的生长,有利于提高烟草产量和品质且经济效益较好.而在复种连作处理下调表达,进而可能使得烟草生长变弱、产量降低、品质变差.
关键词：烤烟；双向电泳；蛋白质组学
中图分类号：TS413 文章编号：1007-5119（2014）01-0089-07DOI：10.13496/j.issn.1007-5119.2014.01.017
Analysis of Root Differential Expression Proteins of Nicotiana Tabacum under Two Planting Systems
YOU Chuihuai1,2, CHEN Dongmei1,2, HUANG Jinwen1, TANG Lina3, XU Zhibing1,2,
ZHANG Zhongyi1,2, LIN Wenxiong1,2*
(1. Agro-ecological Institute, Fujian Agricultural and Forestry University, Fuzhou 350002, China;
2. Institute of Tobacco, Fujian Agricultural and Forestry University, Fuzhou 350002, China;
3. Tobacco Agricultural Science Research Institute of Fujian Province, Fuzhou 350003, China)
Abstract: Root differential proteomics was employed in this study to explore the impact of different planting patterns (multiple cropping rotation (MR) and consecutive monoculture multiple cropping (MC) modes) on growth of flue-cured tobacco (Nicotina tobaccum L). The results showed that a total of 15 protein spots had significant expression difference; 14 protein spots were identified by LC-MS/MS analysis and database searching. Among which, the function of 11 proteins were identified, three proteins were related to the aroma formation, i.e. transketolase, monodehydroascorbate reductase(MDHA), O-methyltransferase-like protein were up-regulated under MR, it was helpful probably to enhance the tobacco quality. While under the MC treatment, they were down-regulated, and the tobacco quality could be worse. Phosphoglycerate kinase, mitochondrial import inner membrane translocase subunit Tim13, NBS-LRR(nucleotide binding site plus leucine-rich repeat), DnaJ protein, Vesicle trafficking protein Sly1, Ribosomal protein-like protein were involved in energy metabolism, plant antioxidation reaction, translocation of solute, metabolism of nucleic acid, respectively. They were very important for the growth development of tobacco and all up-regulated under MR treatments, probably increasing the yield and improving the quality of tobacco, and gaining better economic benefit. They were all down-regulated under MC, which may cause weak growth, low yield and poor quality of tobacco.
Keywords: flue-cured tobacco; two-dimensional electrophoresis; differential proteomic
基金项目：福建省烟草公司项目“福建烟区以烟为主耕作制度研究”{闽烟合同[2006]18号},“福建清香型烟叶适宜区生态基础研究”（闽烟合同[ 2010]-036）
作者简介：尤垂淮,男,博士研究生,研究方向为烟草生理生态和栽培技术.E-mail：you123chui@163.com.*通信作者,E-mail：wenxiong181@163.com
收稿日期：2013-01-05 修回日期：2013-10-10
烟草属忌连作作物,生产上因单种连作或复种连作都存在连作障碍.李天福等[1]研究表明,种植烟草的间隔时间越短,发病率越高,而且烟草花叶病、烟草黑胫病、根黑腐病、根结线虫病、青枯病、赤星病、炭疽病、角斑病和低头黑等的发病率都和连作年限呈不同程度的正相关.由于烟草各种病害发生,造成产量和品质明显下降[2],造成巨大的经济损失.据初步估计,每年全国烟叶10%~15%的损失由各种病害造成的,严重时高达70%~90%,甚至绝收[3].当前,我国因烟草连作,每年直接及间接经济损失高达40亿元[4],烟草可持续发展已经受到严重威胁,严重影响烟草的生产和区域经济的发展.有关烟草连作障碍的研究还处于初步阶段,分子机理方面尚未报道,需深入研究.
结论：关于对不知道怎么写烤烟论文范文课题研究的大学硕士、相关本科毕业论文种植什么最赚钱论文开题报告范文和文献综述及职称论文的作为参考文献资料下载。
小议英汉思维和表达方式的差异
语言学家吕叔湘先生在《中国人学英语》中谈道：“对于中国学生，最有用的帮助是让他认识到英语和汉语的差别……让他们通过比较得到更深刻的领会。” 由此。
种植密度对烤烟产质量影响
摘 要：以烤烟品种云烟85为试验材料，研究了不同种植密度对其生育期、农艺性状、质量、产量等方面的影响。结果表明： 种植密度对烤烟生育期影响不大，。
不同地膜覆盖种植方式和施肥对旱地春玉米生产影响
摘 要：试验设置普通膜、生物膜、液膜和不覆膜四种处理，对玉米苗的出苗、生长、产量和水分利用效率的影响。结果表明，生物地膜覆盖条件下的每个指标和常。
股东表决权行使方式其路径表达
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